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blunt end cloning vector pjet1 2  (Addgene inc)


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    Structured Review

    Addgene inc blunt end cloning vector pjet1 2
    Blunt End Cloning Vector Pjet1 2, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/flag-pcdna3/NHR1-Flag+pcDNA3%2E1+(Plasmid+%2317315)/pmc12804166-56-8-12
    Average 94 stars, based on 2 article reviews
    blunt end cloning vector pjet1 2 - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    Generated:

    Article Title: SMAD3 promotes expression and activity of the androgen receptor in prostate cancer
    Article Snippet: SMAD3 truncation mutants (ΔN, lacking the MH1 domain (133–425) or ΔC, lacking the MH2 domain (1–225)) were generated by PCR and subcloned into Flag-pcDNA3. pLV hU6-sgRNA hUbC-dCas9-KRAB-T2a-Puro plasmid was a gift of Dr Charles Gersbach (Addgene plasmid #71236). shRNAs in pLKO.1 vector targeting SMAD3 (TRCN0000330127, TRCN0000330128), SMAD2 (TRCN0000010477) and SMAD4 (TRCN0000040031) were from Sigma-Aldrich (St. Louis, MO).

    Polymerase Chain Reaction:

    Article Title: SMAD3 promotes expression and activity of the androgen receptor in prostate cancer
    Article Snippet: SMAD3 truncation mutants (ΔN, lacking the MH1 domain (133–425) or ΔC, lacking the MH2 domain (1–225)) were generated by PCR and subcloned into Flag-pcDNA3. pLV hU6-sgRNA hUbC-dCas9-KRAB-T2a-Puro plasmid was a gift of Dr Charles Gersbach (Addgene plasmid #71236). shRNAs in pLKO.1 vector targeting SMAD3 (TRCN0000330127, TRCN0000330128), SMAD2 (TRCN0000010477) and SMAD4 (TRCN0000040031) were from Sigma-Aldrich (St. Louis, MO).

    Plasmid Preparation:

    Article Title: SMAD3 promotes expression and activity of the androgen receptor in prostate cancer
    Article Snippet: SMAD3 truncation mutants (ΔN, lacking the MH1 domain (133–425) or ΔC, lacking the MH2 domain (1–225)) were generated by PCR and subcloned into Flag-pcDNA3. pLV hU6-sgRNA hUbC-dCas9-KRAB-T2a-Puro plasmid was a gift of Dr Charles Gersbach (Addgene plasmid #71236). shRNAs in pLKO.1 vector targeting SMAD3 (TRCN0000330127, TRCN0000330128), SMAD2 (TRCN0000010477) and SMAD4 (TRCN0000040031) were from Sigma-Aldrich (St. Louis, MO).



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